Overview

A panel of innovative investigators presents, in readily reproducible detail, the latest techniques for gene replacement, gene knockout, and gene repair in both plants and animals. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), discuss the parameters that affect vector uptake, and detail successful methods for gene targeting (Cre-ox, and adeno and adeno-associated viruses). The methods include two fundamentally different approaches to gene targeting - one uses a fusion protein to deliver a vector to a virus for possible therapeutic effect, and the other entails an episomal-based Epstein-Barr vector that can modulate the chromatin assembly process, and thus overcome a serious barrier to therapeutic gene targeting. The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field. Gene Targeting Protocols offers today's researchers robust and proven gene targeting techniques that are essential to understanding biological processes at the genetic level.

Full Product Details

Author:   Eric B. Kmiec
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   133
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   1.230kg
ISBN:  

9780896033603

ISBN 10:   0896033600
Pages:   233
Publication Date:   23 September 1999
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Nucleic Acid Transfer Using Cationic Lipids.- Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function.- Gene Transfer and Drug Delivery by Electronic Pulse Delivery.- Strategies for Improving the Frequency and Assessment of Homologous Recombination.- Effective Gene Transfer Using Viral Vectors Based on SV40.- Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase.- Site-Directed Alteration of Genomic DNA by Small-Fragment Homologous Replacement.- Mutation Correction by Homologous Recombination with an Adenovirus Vector.- Site-Specific Targeting of DNA Plasmids to Chromosome 19 Using AAV Cis and Trans Sequences.- Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle.- Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vector, Directed Against High-Affinity ?-Chain of Human Interleukin-2 Receptor.- EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells.- Triplex-Directed Site-Specific Genome Modification.- Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylating Oligodeoxynucleotides.- Gene Targeting in Plants via Site-Directed Mutagenesis.- Antisense Oligonucleotides as Modulators of Pre-mRNA Splicing.

Reviews

"""The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field."" - Cellular and Molecular Biology"

The book also has several techniques for using oligonucleotides in gene targeting, a methodology that may well revolutionize the entire field. - Cellular and Molecular Biology

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