Overview

Full Product Details

Author:   Muhammad Sohail (University of Oxford, UK) ,  Muhammad Sohail (University of Oxford, UK University of Oxford, UK Univ. of Oxford Univ. of Oxford Univ. of Oxford University of Oxford University of Oxford, UK) ,  M. Barciszewska-Pacak ,  Olivier Donze (Apotech Corporation, Epalinges, Switzerland)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Dimensions:   Width: 15.60cm , Height: 2.50cm , Length: 23.40cm
Weight:   0.703kg
ISBN:  

9780849321412

ISBN 10:   0849321417
Pages:   398
Publication Date:   27 August 2004
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Tertiary & Higher Education
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Gene silencing by RNA interference and the role of small interfering RNAs. Basics of siRNA design and chemical synthesis. Oligonucleotide scanning arrays in the design of small interfering RNAs. siRNA production by in vitro transcription. Production of siRNAs with the application of deoxyribozymes. Production of siRNA in vitro by enzymatic digestion of double-stranded RNA. Plasmid-mediated intra cellular expression of siRNAs. Lentiviral vector-mediated delivery of si/shRNA. Exogenous siRNA delivery: protocols for optimising delivery to cells. RNAi in Drosophila cell cultures RNAi in Caenorhabditis elegans. Delivery of RNAi reagents in C. elegans by microinjection. RNAi in Xenopus laevis oocytes. RNAi in Xenopus embryos. An RNAi-based genomic library for forward genetics in the African trypanosome. RNAi in the malaria parasite Plasmodium. Silencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches. RNAi in transgenic animal models. RNA interference in mouse model. Virus-induced RNA silencing in plants. RNA silencing in plants - biolistic delivery of RNAi reagents. Agro-infiltration: a versatile tool for RNAi studies in plant.

Reviews

Author Information

Muhammad Sohail

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions