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OverviewThe preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals. Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes Full Product DetailsAuthor: B. D. Hames , S. J. Higgins (both at the Department of Biochemistry and Molecular Biology, University of Leeds)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 161 Dimensions: Width: 15.70cm , Height: 1.60cm , Length: 23.40cm Weight: 0.506kg ISBN: 9780199634002ISBN 10: 0199634009 Pages: 284 Publication Date: 12 October 1995 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of ContentsIntroduction 1: Strategies for using gene probes 2: Preparation of nucleic acids for use as probes 3: DNA radiolabelling and detection 4: Non-radioactive labelling of DNA probes 5: Preparation of synthetic oligodeoxynucleotide probes 6: Probe preparation of PCR 7: Synthesis of labelled riboprobes 8: Antibody probes Appendix 1 Appendix 2Reviews`This book is intended for use as a laboratory manual, and contains practical assistance to the best of the current methodology within its subject area.' International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 `a very practical book' Cellular & Molecular Biology, Vol. 42, No. 3, 1996 a very practical book * Cellular & Molecular Biology, Vol. 42, No. 3, 1996 * This book is intended for use as a laboratory manual, and contains practical assistance to the best of the current methodology within its subject area. * International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 * This book is intended for use as a laboratory manual, and contains practical assistance to the best of the current methodology within its subject area. International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 a very practical book Cellular & Molecular Biology, Vol. 42, No. 3, 1996 Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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