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OverviewGene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutations. A companion volume covers the preparation of these probes. The volumes aim to contribute to a growing field of research. Full Product DetailsAuthor: B. D. Hames , S. J. Higgins (both at the Department of Biochemistry and Molecular Biology, University of Leeds)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 162 Dimensions: Width: 15.60cm , Height: 2.30cm , Length: 23.40cm Weight: 0.687kg ISBN: 9780199634149ISBN 10: 0199634149 Pages: 446 Publication Date: 23 November 1995 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of Contents1: Hybridization strategy 2: Screening recombinant DNA libraries by hybridization and amplification 3: Screening human recombinant libraries with nucleic acid probes 4: Antibody screening of bacterial expression systems 5: Isolation of cDNA clones by direct expression in mammalian cells 6: Genomic mapping and gene structure analysis 7: Analysis of gene expression by blotting techniques 8: In situ hybridization of nucleic acid probes to cellular RNA 9: Applications of chromosomal in situ hybridization 10: Analysis of gene expression analysis by PCR: a quantitative approach 11: Analysis of protein expression by immunocytochemistry 12: Gene targeting in mammalian cells 13: Directed mutagenesis and mutant analysis 14: Human gene mutational analysis Appendix 1 Appendix 2ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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