Overview

Highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for the analysis of the mouse phenotype. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today.

Full Product Details

Author:   Martin J. Tymms ,  Ismail Kola
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   158
Dimensions:   Width: 15.20cm , Height: 2.80cm , Length: 22.90cm
Weight:   0.826kg
ISBN:  

9780896035720

ISBN 10:   0896035727
Pages:   431
Publication Date:   15 January 2001
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Overview.- Isolation and Maintenance of Primate Embryonic Stem Cells.- Gene Targeting in ES Cells.- Manipulating Mouse Embryonic Stem Cells.- Gene Targeting in a Centralized Facility.- The LoxP/CRE System and Genome Modification.- Creation and Use of a Cre Recombinase Transgenic Database.- Choice of Mouse Strains for Gene Targeting.- Isolation, Microinjection, and Transfer of Mouse Blastocysts.- Aggregation Chimeras.- How to Study Pathologic Phenotypes of Knockout Mice.- Analysis of Hematopoietic Phenotypes in Knockout Mouse Models.- Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function.- Influence of Genetic Background on Knockout Mouse Phenotypes.- Lineage Allocation During Early Embryogenesis.- Generation of Double-Knockout Embryonic Stem Cells.- In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes.- Embryonic Stem Cells in the Study of Hematopoiesis.- Interferon-Inducible ES Cell Expression Systems.- Transgenic Studies in the Mouse.- In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping.- Epigenetic Effects on Transgene Expression.- Positional-Candidate Cloning of Genes from Mouse Mutants.- Genetically Engineered Mice.- Embryo Cryopreservation for Transgenic Mouse Lines.

Reviews

This is a valuable reference work, which will no doubt find a home on many lab benches. -E-STREAMS

This is a valuable reference work, which will no doubt find a home on many lab benches. - E-STREAMS

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