Overview

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.

Full Product Details

Author:   Richard A. Shimkets
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   258
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   0.475kg
ISBN:  

9781588292209

ISBN 10:   1588292207
Pages:   168
Publication Date:   24 February 2004
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Technical Considerations in Quantitating Gene Expression.- Gene Expression Quantitation Technology Summary.- Standardized RT-PCR and the Standardized Expression Measurement Center.- GeneCalling.- Invader Assay for RNA Quantitation.- Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays.- Amplified Differential Gene Expression Microarray.- Suppression Subtractive Hybridization.- Small Amplified RNA-SAGE.- Gene Expression Informatics.

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