Overview

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics.

Full Product Details

Author:   William C. Heiser
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   246
Dimensions:   Width: 15.50cm , Height: 3.10cm , Length: 23.50cm
Weight:   1.147kg
ISBN:  

9781588290953

ISBN 10:   1588290956
Pages:   566
Publication Date:   28 October 2003
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Delivery Using Adenoviruses.- Adenovirus-Mediated Gene Delivery.- DNA Delivery to Cells in Culture.- Adenovirus-Mediated Gene Delivery to Skeletal Muscle.- Delivery of Adenoviral DNA to Mouse Liver.- Delivery of DNA to Lung Airway Epithelium.- Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors.- Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors.- Adenovirus-Mediated Gene Transfer to Tumor Cells.- Adenovirus-Mediated Gene Delivery to Dendritic Cells.- Delivery Using Adeno-Associated Viruses.- Overview of Adeno-Associated Viral Vectors.- AAV Vector Delivery to Cells in Culture.- AAV-Mediated Gene Transfer to Skeletal Muscle.- AAV-Mediated Gene Transfer to the Liver.- AAV-Mediated Gene Transfer to Mouse Lungs.- Gene Delivery to the Mammalian Heart Using AAV Vectors.- Gene Delivery to the Mouse Brain with Adeno-Associated Virus.- Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus.- Gene Delivery to Human and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors.- Delivery Using Herpes Simplex Viruses.- Delivery Using Herpes Simplex Virus.- Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors.- Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System.- Gene Transfer to Glial Tumors Using Herpes Simplex Virus.- Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells.- Delivery Using Baculoviruses.- Baculovirus-Mediated Gene Delivery into Mammalian Cells.- Delivery Using Lentiviruses.- Gene Delivery by Lentivirus Vectors.- Lentiviral Vectors for the Delivery of DNA into Mammalian Cells.- Stable Gene Delivery to CNS Cells Using Lentiviral Vectors.- Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors.- Delivery of Genes to the Eye Using Lentiviral Vectors.- Lentiviral Transduction of Human Dendritic Cells.- Delivery Using Retrovirus.- Gene Transfer by Retroviral Vectors.- Gene Delivery to Cells in Culture Using Retroviruses.- Retrovirus-Mediated Gene Transfer to Tumors.- Dilivery of Genes to Hematopoietic Stem Cells.- Delivery Using Alphaviruses.- Delivery and Expression of Heterologous Genes in Mammalian Cells Using Self-Replicating Alphavirus Vectors.

Reviews

For Volumes 1 & 2 This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike. - Medical Oncology ...clearly and succinctly presented...a must for any biology or medical library. - ASM News

For Volumes 1 & 2 This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike. Medical Oncology

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