Fusarium wilt: Methods and Protocols

Author:   Jeffrey Coleman
Publisher:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2391
ISBN:  

9781071617977


Pages:   208
Publication Date:   24 October 2022
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Fusarium wilt: Methods and Protocols


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Author:   Jeffrey Coleman
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2391
Weight:   0.426kg
ISBN:  

9781071617977


ISBN 10:   1071617974
Pages:   208
Publication Date:   24 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.  Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates Kerry O’Donnell, Imana Laraba, and David M. Geiser   2.  Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing Zunyong Liu, Eun-Gyu No, Catherine Danmaigona Clement, Ping He, and Libo Shan   3.  Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method Yu Ayukawa and Masatoki Taga   4.  RNA-seq data analysis for differential expression Navdeep Gill and Braham Dhillon   5.  Quantification and isolation of spontaneous colony growth variants Cristina López Díaz, Lucía Gómez-Gil, Elena Pérez-Nadales, Gesabel Navarro Velasco, and Antonio Di Pietro   6.  Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens Yan-Hong Dong and Shu-Tong Wang   7.  Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum Ambika Pokhrel, Seungyeon Seo, Qiang Wang, and Jeffrey J. Coleman   8.  CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum Qiang Wang and Jeffrey J. Coleman   9.  In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin Tânia R. Fernandes, Antonio Serrano, and Antonio Di Pietro   10.  Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria Ningxiao Li and Seogchan Kang   11.  Isolation of mitochondria from Fusarium species Michael MacKillop and John C. Kennell   12.  In planta gene expression analysis and colonization of Fusarium oxysporum Virginia Casado-del Castillo, Ernesto Pérez Benito, and José María Díaz-Mínguez   13.  Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots Hye-Seon Kim, Sook-Young Park, Seogchan Kang, and Kirk Czymmek   14.  High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum Yong Zhang, Pei-Lun Kao, Akaansha Rampal, Sibongile Mafu, Sergey N. Savinov, and Li-Jun Ma   15.  Screening and assessment of pisatin demethylase activity (PDA) Catherine C. Wasmann and Jeffrey J. Coleman   16.  Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton Jinggao Liu and Tanya A. Wagner

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