Overview

This volume brings together practical techniques for detecting and measuring free radicals - the reactive, short-lived molecules now known to play an important role in a large number of human diseases, including heart disease and cancer - and for studying their effects. Consequently, their study in biological systems has increased enormously in recent years, and this book may be of interest to a wide cross-section of biochemists and clinicians.

Full Product Details

Author:   Neville A. Punchard (Department of Biological and Health Sciences, University of Luton) ,  Frank J. Kelly (The Rayne Institute, St Thomas's Hospital, London)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   168
Dimensions:   Width: 15.60cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.580kg
ISBN:  

9780199635597

ISBN 10:   0199635595
Pages:   334
Publication Date:   22 August 1996
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Introduction 1: Direct and indirect measurements of free radicals by physico-chemical methods 1.1: In vitro and in vivo detection of free radicals metabolites with electron spin resonance 1.2: The application of high-field nuclear magnetic resonance spectroscopy to the detection of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs 2: Direct and indirect measurement of free radicals by other methods 2.1: Intrinsic (low-level) chemiluminescence 2.2: Visual assessment of oxidative stress by multifunctional digital microfluorography 2.3: Salicylic acid and phenylalanine as probes to detect hydroxyl radicals 3: Measurement of free radical products 3.1: Lipids (i): peroxides and other products 3.2: Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal 3.3: Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipid peroxidation 3.4: Proteins (i): determination of carbonyl groups in oxidized proteins 3.5: Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA 3.6: Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems 3.7: Nucleic acids: measurement of products of free radical attack on nucleic acids 4: Measurement of antioxidants 4.1: Glutathione 4.2: Glutathione peroxidase: activity and steady-state level of mRBA 4.3: Superoxide dismutase 4.4: Application of deuterium labelling and gas chromatography-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids 4.5: Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants 4.6: Measurement of antioxidant gene expression Appendix

Reviews

Introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts for which the protocols are relevant...This book will be not just a laboratory manual but should be a bestseller in the student market. --Times Higher Education Supplement<br>

Introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts for which the protocols are relevant...This book will be not just a laboratory manual but should be a bestseller in the student market. --Times Higher Education Supplement

`will greatly assist any laboratory wishing to enter the fray in what must be one of the most exciting areas of medicine, nutritional science and food technology ... the introductory sections to each chapter are in themselves small masterpieces of explanation of the states of the arts for which the protocols are relevant. Thus this book will be not just a laboratory manual but should be a bestseller in the student market.' Times Higher Education Supplement

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