Overview

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction.  Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.

Full Product Details

Author:   Jin Zhang ,  Qiang Ni ,  Robert H Newman
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2014
Volume:   1071
Dimensions:   Width: 17.80cm , Height: 1.40cm , Length: 25.40cm
Weight:   5.018kg
ISBN:  

9781493960149

ISBN 10:   1493960148
Pages:   251
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

The Design and Application of Genetically-Encodable Biosensors Based on Fluorescent Proteins.- An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications.- Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells using Genetically Encoded Fluorescent Biosensors.- Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells.- Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters.- Genetically Encoded Fluorescent Biosensors for Live-cell Imaging of Lipid Dynamics.- Live Cell Imaging of Cytosolic NADH-NAD+ Redox State using a Genetically Encoded Fluorescent Biosensor.- Measuring Membrane Voltage with Microbial Rhodopsins.- Imaging the Activity of Ras superfamily GTPase Proteins in Small Subcellular Compartments in Neurons.- Imaging Kinase Activity at Protein Scaffolds.- Using a Genetically Encoded FRET-Based Reporter to Visualize calcineurin phosphatase Activity in Living Cells.- Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation.- Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity.- Biosensor Imaging in Brain Slice Preparations.- Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster.- Multi-Parameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel.- FRET and BRET-Based Biosensors in Live Cell Compound Screens.- Integrating Fluorescent Biosensor Data Using Computational Models,

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions