Overview

This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.

Full Product Details

Author:   Christopher P. Toseland ,  Natalia Fili
Publisher:   Springer Basel
Imprint:   Springer Basel
Edition:   2014 ed.
Volume:   105
Dimensions:   Width: 15.50cm , Height: 1.90cm , Length: 23.50cm
Weight:   5.856kg
ISBN:  

9783034808552

ISBN 10:   3034808550
Pages:   298
Publication Date:   19 August 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Fluorescence and Labelling: How to choose and what to do.- Fluorescent biosensors: design and application to motor proteins.- Rapid Reaction Kinetic Techniques.- Fluorescence to study the ATPase mechanism of Motor Proteins.- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies.- Fluorescent methods to study transcription initiation and transition into elongation.- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo.- Fluorescence methods in the investigation of the DEAD-box helicase mechanism.- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins.- Fluorescence Tracking of Motor proteins in vitro.- Measuring Transport of Motor Cargos.- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET.- Using fluorescence to study actomyosin in yeasts.

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