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Author:   J. Slavík
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   Softcover reprint of the original 1st ed. 1996
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   0.504kg
ISBN:  

9781489918680

ISBN 10:   148991868
Pages:   306
Publication Date:   12 June 2013
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Fluorescence Microscopy and Fluorescent Probes.- Fluorescence Microscopy: State of the Art.- Fluorescence Lifetime-Resolved Imaging Microscopy: A General Description of Lifetime-Resolved Imaging Measurements.- Confocal Fluorescence Lifetime Imaging.- Multidimensional Fluorescence Microscopy: Optical Distortions in Quantitative Imaging of Biological Specimens.- Fluorescent Probes.- Flow Cytometry versus Fluorescence Microscopy.- Multichannel Fluorescence Microscopy and Digital Imaging: On the Exciting Developments in Fluorescence Microscopy.- Fluorescence Lifetime Imaging and Spectroscopy in Photobiology and Photomedicine.- A Versatile Time-Resolved Laser Scanning Confocal Microscope.- Ion-Sensitive Fluorescent Probes.- Disappearance of Cytoplasmic Ca2+ Oscillations Is a Sensitive Indicator of Photodamage in Pancreatic ?-Cells.- Distribution of Individual Cytoplasmic pH Values in a Cell Suspension.- The Effect of Lysosomal pH on Lactoferrin-Dependent Iron Uptake in Tritrichomonas foetus.- On the Protein-Error of the Calcium-Sensitive Fluorescent Indicator Fura-Red.- Cytoplasmic Ion Imaging: Evidence for Intracellular Calibration Heterogeneities of Ion-Sensitive Fluoroprobes.- The Effect of Protein Binding on the Calibration Curve of the pH Indicator BCECF..- Artifacts in Fluorescence Ratio Imaging.- Use of Fluorescent Probes and CLSM for pH-Monitoring in the Whole Plant Tissue: pH Changes in the Shoot Apex of Chenopodium rubrum Related to Organogenesis.- Spatial Resolution of Cortical Cerebral Blood Flow and Brain Intracellular pH as Measured by in Vivo Fluorescence Imaging.- Membrane Potential-Sensitive Fluorescent Probes.- Is a Potential-Sensitive Probe diS-c3(3) a Nernstian Dye?: Time-Resolved Fluorescence Study with Liposomes as a Model System.- Kinetic Behavior ofPotential-Sensitive Fluorescent Redistribution Probes: Modelling of the Time Course of Cell Staining.- Speed of Accumulation of the Membrane Potential Indicator dis-c3(3) in Yeast Cells.- Spectral Effects of Slow Dye Binding to Cells and Their Role in Membrane Potential Measurements.- Exploitation of Rhodamine B in the Killer Toxin Research.- Fluorescent Probes for Nucleic Acids.- “In Situ” Estimates of the Spatial Resolution for “Practical” Fluorescence Microscopy of Cell Nuclei.- Requirements for a Computer-Based System for FISH Applications.- Fluorescent Dyes and Dye Labelled Probes for Detection of Nucleic Acid Sequences in Biological Material.- Fluorescence in Situ Hybridization (FISH) in Cytogenetics of Leukemia.- Estimation of “Start” in Saccharomyces cerevisiae by Flow Cytometry and Fluorescent Staining of DNA and Cell Protein.- Fluorescence Image Cytometry of DNA Content: A Comparative Study of Three Fluorochromes and Four Fixation Protocols.- Fluorescent Labels, Fluorescent and Fluorogenic Substrates.- In Vivo Tissue Characterization Using Environmentally Sensitive Fluorochromes.- Sensitive and Rapid Detection of ß-Galactosidase Expression in Intact Cells by Microinjection of Fluorescent Substrate.- Fluorogenic Substrates Reveal Genetic Differences in Aldehyde-Oxidating Enzyme Patterns in Rat Tissues.- Binding of Prothrombin Fragment 1 to Phosphatidylserine Containing Vesicles: A Solvent Relaxation Study.- New Thiol Active Fluorophores for Intracellular Thiols and Glutathione Measurement.- Quantification of Macrophages in the Cardiovascular System of Hypercholesterolemic Rabbits by Use of Digital Image Processing.- Fluorescence Assay for Studying P-Glycoprotein Function at Single Cell Level.- Alterations of Vimentin-Nucleus Interactions as anEarly Phase in Cholesterol Oxide-Induced Endothelial Cell Damage.- Fluorescence Microscopy of Rye Cell Walls from Kernels to Incubated Doughs.- Practical Approach for Immunohistochemical Staining of Muscle Biopsies.- Digital Image Analysis.- Rapid Automatic Segmentation of Fluorescent and Phase-Contrast Images of Bacteria.- Use of Confocal Microscopy for Absolute Measurement of Cell Volume and Total Cell Surface Area.- Cell Volume Measurements Using Confocal Laser Scanning Microscopy.- Subcellular Cytofluorometry in Confocal Microscopy.- Application of Confocal Microscopy to 3-D Reconstruction and Morphometrical Analysis of Capillaries.- Retrieving Spatio Temporal Information from Confocal Data: A Study Using Melanotrope Cells of Xenopus laevis.- Dynamics of Actin Measured by Fluorescence Correlation Microscopy (FCM).

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