Overview

Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts. The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations. The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine.

Full Product Details

Author:   Richard B. Thompson ,  Richard B. Thompson ,  Douglas A. Christensen (University of Utah, Salt Lake City, USA) ,  Weihong Tan (University of Florida, Gainesville, Florida, USA)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Dimensions:   Width: 15.60cm , Height: 2.70cm , Length: 23.40cm
Weight:   0.725kg
ISBN:  

9780824727376

ISBN 10:   0824727371
Pages:   420
Publication Date:   16 December 2005
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Reviews

... emphasizes the most recent developments and emerging technologies with the broadest impacts in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high throughput screening, and clinical diagnostics. Roughly grouped under three headings, namely new recognition or transduction approaches, other new technology, and selected applications, ...The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, ... provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine. -- J Albaiges, Editor in Chief, Department of Environment Chemistry CID-CSIC, Barcelona, Spain, in International Journal Of Environmental Analytical Chemistry, Vol. 86, 2006 Written by internationally renowned authors in their fields, this book provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine. - In Anticancer Research, July/ August 2008, Vol. 28, No. 4A

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Richard B.Thompson

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