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OverviewFlow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering. Full Product DetailsAuthor: Larry A. Sklar (Regents' Professor of Pathology, Regents' Professor of Pathology, University of New Mexico)Publisher: Oxford University Press Inc Imprint: Oxford University Press Inc Dimensions: Width: 23.60cm , Height: 2.30cm , Length: 16.00cm Weight: 0.672kg ISBN: 9780195183146ISBN 10: 0195183142 Pages: 400 Publication Date: 22 September 2005 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: To order  Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of ContentsReviewsFrom the material presented here, there can be no doubt that flow cytometry is a technology that is tremendously adaptable and can advance almost any area of biotechnology. This volume should appeal to anyone who is fascinated by this technology and its many varied scientific applications. -- Quarterly Review of Biology """From the material presented here, there can be no doubt that flow cytometry is a technology that is tremendously adaptable and can advance almost any area of biotechnology. This volume should appeal to anyone who is fascinated by this technology and its many varied scientific applications.""-- Quarterly Review of Biology" Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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