Overview

This collection of the most important methodologies for the analysis of the structure and function of flavoproteins ranges from wet to dry chemistry. These step-by-step methods - developed by leading experimentalists to work reliably - include the most commonly used techniques as well as a wide range of spectrophotometric protocols that allow direct monitoring of flavins in real time, in the steady state, or as reaction intermediates. Among the techniques detailed are freeze-quench methods, EPR spectroscopy, CD spectroscopy, vibrational spectroscopy (SERRS), and nuclear magnetic resonance. Also discussed are a variety of therapeutic applications for flavins and flavoproteins that go far beyond the use of riboflavin as a vitamin supplement. Flavoprotein Protocols offers to both experts in the field and to those working on a flavin-containing protein for the first time a wealth of proven methods and laboratory experience that will enhance their understanding of structure/function relationships in the proteins and of the effects of the protein environment on flavin chemistry.

Full Product Details

Author:   Steven K. Chapman ,  Graeme A. Reid
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   131
Dimensions:   Width: 15.50cm , Height: 1.50cm , Length: 23.50cm
Weight:   0.454kg
ISBN:  

9780896037342

ISBN 10:   0896037347
Pages:   256
Publication Date:   01 July 1999
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
We will order this item for you from a manufatured on demand supplier.

Table of Contents

UV-Visible Spectroscopy as a Tool to Study Flavoproteins.- Identifying and Quantitating FAD and FMN in Simple and in Iron-Sulfur-Containing Flavoproteins.- Fluorescence Analysis of Flavoproteins.- Potentiometric Measurement of Oxidation-Reduction Potentials.- Flavoprotein Kinetics.- Application of Freeze Quenching to the Study of Rapid Reactions in Flavoproteins.- Application of Electron Spin Resonance (ESR) for Detection and Characterization of Flavoprotein Semiquinones.- Circular Dichroism Studies of Flavoproteins.- Vibrational Spectroscopy of Flavoproteins.- NMR of Flavoproteins.- Flavoprotein Resolution and Reconstitution.- Flavoenzyme Structure and Function.- Identification of Covalent Flavoproteins and Analysis of the Covalent Link.- Flavin Synthesis and Incorporation into Synthetic Peptides.- Computational Methods in Flavin Research.- Flavins and Flavoenzymes in Diagnosis and Therapy.

Reviews

A detailed analysis of flavoproteins will be of value for investigators working with flavin enzymes whether studying mechanisms or searching for inhibitors. Chapters on chemical syntheses and the use of analogs provide useful information on approaches to both of these areas.. -Doodys Health Sciences Book Review Journal

A detailed analysis of flavoproteins will be of value for investigators working with flavin enzymes whether studying mechanisms or searching for inhibitors. Chapters on chemical syntheses and the use of analogs provide useful information on approaches to both of these areas. - Doodys Health Sciences Book Review Journal

"""A detailed analysis of flavoproteins will be of value for investigators working with flavin enzymes whether studying mechanisms or searching for inhibitors. Chapters on chemical syntheses and the use of analogs provide useful information on approaches to both of these areas..""-Doodys Health Sciences Book Review Journal"

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