Overview

Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.

Full Product Details

Author:   Bernd W. Rautenstrauss ,  Thomas Liehr
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   2002 ed.
Dimensions:   Width: 19.30cm , Height: 2.10cm , Length: 24.20cm
Weight:   1.160kg
ISBN:  

9783540672760

ISBN 10:   3540672761
Pages:   504
Publication Date:   14 February 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Book
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

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Reviews

From the reviews: Ever wondered how to catch your FISH ... in the dark pools of cellular biology? Then ... FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) ... . Every chapter is illustrated by several schematic drawings ... . The manual is completed by a subject index ... . The book is certainly clear ... providing valuable protocols for both beginning FISHers and for FISH experts. (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

From the reviews: <p> Ever wondered how to catch your FISH a ] in the dark pools of cellular biology? Then a ] FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) a ] . Every chapter is illustrated by several schematic drawings a ] . The manual is completed by a subject index a ] . The book is certainly clear a ] providing valuable protocols for both beginning FISHers and for FISH experts. (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)

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