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OverviewThis convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labelling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics techniques in current use. This book will be of particular value to scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics. Full Product DetailsAuthor: Barbara G Beatty (Health Sciences Research Facility, University of Vermont, USA) , Sabine Mai (Manitoba Institute of Cell Biology, Winnipeg, Canada) , Jeremy A Squire (Ontario Cancer Institute, Toronto, Canada)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 260 Dimensions: Width: 19.00cm , Height: 1.70cm , Length: 24.60cm Weight: 0.671kg ISBN: 9780199638840ISBN 10: 0199638845 Pages: 274 Publication Date: 04 July 2002 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: Out of stock  Table of Contents1: Beatty, Mai, Squire: Introduction 2: Bray-Ward: Labelling Methods 3: Beatty, Scherer: FISH Mapping 4: Mai, Wiener: Murine FISH 5: Heng: Fiber FISH 6: Tam et al: RNA FISH 7: Cremer et al: Three Dimensional FISH 8: Lichter, Joos, Schwanen: CGH 9: Squire, Marrano, Kolomietz: Clinical Cytogenetics 10: Bayani, Squire: Multicolour FISH and SKY 11: Khan et al: cDNA Microarrays and FISHReviewsAs a resource for both the researcher and the clinician, this book should serve admirably. It should be a welcome addition to any technical library. Proteomics ... offers an excellent array of protocols aimed at the laboratory scientist. Proteomics ... the book does an excellent job of describing purification and enzymatic labeling techniques of probe DNA, and offers a comprehensive list of sources for various FISH probes and reagents. Proteomics ... useful as a laboratory manual. Proteomics Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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