Overview

This volume explores current technologies used to investigate the formation, insertion, and function of metalloclusters associated with proteins. Chapters describe relevant topics about Fe-S cluster metabolism are explored through genetic, biochemical, spectroscopic methods.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Fe-S Proteins: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Full Product Details

Author:   Patricia C. Dos Santos
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2353
Weight:   0.692kg
ISBN:  

9781071616079

ISBN 10:   1071616072
Pages:   351
Publication Date:   24 July 2022
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

  Part I - Methods for genetic manipulation and Fe-S protein expression 1. Molecular biology and genetic tools to investigate functional redundancy among Fe-S cluster carriers in E. coli Yohann Duverger and Béatrice Py   2. Study Fe-S cluster proteins in Methanococcus maripaludis, a model archaeal organism Cuiping Zhao, Christina A Roberts, Ian J Drake, and Yuchen Liu   3. Genetic approaches to uncover gene products involved in iron-sulfur protein maturation: High throughput genomic screening using transposon-sequencing Valerie J. Carabetta, Karla Esquilin-Lebron, Ehud Zelzion, Jeffrey M. Boyd   4. Methods for heterologous overproduction of Fe-S proteins Elliot I. Corless and Edwin Antony   5. Enzymatic and Chemical in vitro Reconstitution of Iron-Sulfur Cluster Proteins Mauro Marengo, Rita Puglisi, Simonetta Oliaro-Bosso, Annalisa Pastore and Salvatore Adinolfi   6. Expression, isolation, and characterization of vanadium nitrogenase from Azotobacter vinelandii Katharina Grunau, Jakob Gies-Elterlein, Christian Trncik and Oliver Einsle   Part II - Biochemical methods to probe steps involved in the synthesis of Fe-S clusters 7.Ni-NTA affinity chromatography to characterize protein-protein interactions during Fe-S cluster biogenesis Terrell D. Carter and F. Wayne Outten   8.  tRNA modifications as a readout of S and Fe-S metabolism Ashley M. Edwards, Maame A. Addo, Patricia C. Dos Santos   9. Methods to unravel the roles of ATPases in Fe–S cluster biosynthesis Christa N. Molé, Kinjal Dave, Deborah L. Perlstein   10. Methods to investigate the kinetic profile of cysteine desulfurases Maame A. Addo, Ashley M. Edwards, Patricia C. Dos Santos 11. A fast and ratiometric method for quantification of cysteine-bound persulfides based on alkylation and gel-shift assays Sylvain Gervason, Batoul Srour, and Benoit D’Autréaux   Part III - Biophysical methods to study Fe-S clusters and reactions promoted by metalloenzymes   12. Circular dichroism to probe the synthesis, transfer, and stability of Fe-S clusters Stefania Iametti, Francesco Bonomi, and Alberto Barbiroli   13. Native mass spectrometry of iron-sulfur proteins Jason C. Crack, and Nick E. Le Brun   14. Characterization of Paramagnetic Iron-Sulfur Clusters using Electron Paramagnetic Resonance Spectroscopy Troy A. Stich 15. Characterization of Fe-S clusters in proteins by Mӧssbauer Spectroscopy Chie Ueda, Michelle Langton, and Maria-Eirini Pandelia   16. Biochemical approaches to probe the role of the auxiliary iron-sulfur cluster of lipoyl synthase from Mycobacterium tuberculosis Vivian Robert Jeyachandran, Jay V. Pendyala, Erin L. McCarthy, Amie K. Boal, and Squire J. Booker 17. Methods to Screen for Radical SAM Enzyme Crystallization Conditions Lydie Martin, Xavier Vernède, and Yvain Nicolet

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