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OverviewThe extracellular matrix surrounds cells and tissues. It provides structure to organisms and is important in determining cell growth, its state of differentiation, and how the cell responds during injury and development. Changes in the extracellular matrix are also important components in both inherited and acquired diseases. Thus, studies of the extracellular matrix span much of modern biological research. Because the extracellular matrix resides outside the cell, the properties of the component molecules require special approaches for their study. This volume describes the majority of basic techniques employed in extracellular matrix research. Full Product DetailsAuthor: M. A. Haralson (Department of Pathology, Vanderbilt University, Nashville, Tennessee) , John R. Hassell (Eye and Ear Institute, University of Pittsburgh, Pennsylvania) , J.R. HassellPublisher: Oxford University Press Imprint: Oxford University Press Volume: 151 Dimensions: Width: 15.50cm , Height: 2.30cm , Length: 23.30cm Weight: 0.742kg ISBN: 9780199632206ISBN 10: 0199632200 Pages: 432 Publication Date: 14 December 1995 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Out of Print Availability: In Print  Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of Contents1: Overview of the different collagen types and approaches for the isolation and characterization of types I, III, V and VI molecules from tissues and cultured cells 2: Approaches for isolating and characterizing the collagens in cartilage 3: Techniques for evaluating the matrix components found in basement membranes 4: Approaches for investigating the specialized matrix components produced by endothelial cells 5: Techniques for the isolation and characterization of the enzymes involved in the post-translational processing of collagen 6: Techniques for the study of fibronectin and tenascin 7: Approaches for the study of glycosaminoglycans 8: Approaches for the evaluation of proteoglycans 9: Techniques for the use of the extracellular matrix and its components in cell culture 10: Approaches for investigating elastin 11: Techniques for evaluating the catabolism of extracellular matrix components 12: Immunological approaches for the identification of extracellular matrix components in tissues and cultured cells 13: Molecular biological approaches for studying extracellular matrix productionReviewsvaluable ... It is a multi-authored volume in the superb Practical Approach series and comes packed with protocols on every aspects of ECM research. This is the ECM book that has been needed for a long time, and here are essential protocols for any groups with even the slightest interest in the ECM. I recommend it highly. Charles Streuli, University of Manchester, Trends in Cell Biology, (Vol.6), November 1996 This book sets out to provide the research worker with a comprehensive survey and detailed protocols of the biochemical techniques used for studying the macromolecules of the extracellular matrix. The senior authors of all the chapters are well established in the field of their contribution. This is a strong recommendation for a book which should become a standard handbook for anyone working in this field, particularly those just entering or wishing to develop new techniques. Doreen E. Ashhurst, St George's Hospital Medical School, Cell Biology International, Vol. 20, No. 9, 1996 `valuable ... It is a multi-authored volume in the superb Practical Approach series and comes packed with protocols on every aspects of ECM research. This is the ECM book that has been needed for a long time, and here are essential protocols for any groups with even the slightest interest in the ECM. I recommend it highly.' Charles Streuli, University of Manchester, Trends in Cell Biology, (Vol.6), November 1996 `This book sets out to provide the research worker with a comprehensive survey and detailed protocols of the biochemical techniques used for studying the macromolecules of the extracellular matrix. The senior authors of all the chapters are well established in the field of their contribution. This is a strong recommendation for a book which should become a standard handbook for anyone working in this field, particularly those just entering or wishing to develop new techniques.' Doreen E. Ashhurst, St George's Hospital Medical School, Cell Biology International, Vol. 20, No. 9, 1996 Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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