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OverviewIRF-2 is a mammalian transcription factor for interferon and interferon inducible genes of having oncogenic activity under certain circumstances.We compared the amino acid sequences of mouse IRF-2 (349 amino acid) with human IRF-2 and found a C-terminal tetra-peptide (314PAPV317) of mouse IRF-2 to be different from (314SSSM317) human IRF-2 (349 amino acid). The murine IRF-2 sequence (PAPV) at 314-317 was replaced by SSSM, the corresponding region of the human IRF-2, by site-directed cassette mutagenesis. Both the wild type and mutant IRF-2 were prepared as GST-fusion proteins expressed in E. coli XL-1 blue cells, which exhibited similar expression patterns and immunoreactivity but different DNA-binding activities with (GAAAGT)4 oligonucleotide. The mutant IRF-2 showed higher complexes than the wild type IRF-2. Also, the amount of the complexes was higher in the mutants. Although the mouse and human IRF-2 are molecularly homologous and physiological similar, replacement of the four amino acid in mouse IRF-2 by the corresponding region from human IRF-2 can influence DNA binding activity of IRF-2. This provides new information on the function of C-terminus of IRF-2. Full Product DetailsAuthor: Krishna Prakash , Rath P.C.Publisher: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Imprint: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Dimensions: Width: 15.20cm , Height: 1.10cm , Length: 22.90cm Weight: 0.307kg ISBN: 9783639329292ISBN 10: 3639329295 Pages: 204 Publication Date: 06 September 2011 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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