Overview

'The book overall is excellent and I recommend it strongly, It is not on my bookshelf, but on the laboratory bench, already in use'R Free Radical Research 'An 'enjoyable and informative book [...] Lane presents a nicely crafted account of an important element's place in our lives. His book deserves to be widely read...' -NatureExperimental Protocols for Free Radical Research is a translated, expanded, and fully updated of the Japanese book Experimental Protocols for Reactive Oxygen Research: assay methods, gene analysis, and pathophysiology models published in 1994. The aim of the book is to provide experimental protocols covering many aspects of free radical research: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained describing the equipment and reagents needed and then detailing the experimental procedure.

Full Product Details

Author:   Naoyuki Taniguchi (Department of Biochemistry, Osaka University Medical School) ,  John Gutteridge (Director of Oxygen Chemistry Laboratory, Royal Brompton and Harefield NHS Trust, London) ,  John M. C. Gutteridge (Director of Oxygen Chemistry Laboratory, Royal Brompton and Harefield NHS Trust, London)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Dimensions:   Width: 16.10cm , Height: 2.40cm , Length: 24.10cm
Weight:   0.749kg
ISBN:  

9780198506683

ISBN 10:   0198506686
Pages:   357
Publication Date:   15 June 2000
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Part One: Measurement ROS and RNS measurements Measurements for proteinaceous plasma antioxidants, low Mw antioxidants, and REDOX related compounds Measurements for specific ROS and RNS enzymes and related proteins Lipids and lipid peroxidization Iron and reactive iron species Copper and reactive copper species Part Two: Gene Analyses Research for gene expressions of ROS and RNS related genes DNA damage and repair Part Three: Experiments for pathophysiological models Isolated and perfused hearts (Langendorf's model) Myocardial ischaemia-reperfusion models in vivo GI tracts in vivo Brain ischaemia Cell culture Athersclerosis Dicarbonyls and HB-EGF gene expression UV damage Ageing Ovulation Scavenging NO in experimental animals Using radioimmuno assays in brain slices Oxidative stress Carcinogenesis Iron overload Copper overload

Reviews

The book overall is excellent and I recommend it strongly, It is not on my bookshelf, but on the laboratory bench, already in use Free Radical Research An enjoyable and informative book [...] Lane presents a nicely crafted account of an important element's place in our lives. His book deserves to be widely read... Nature

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