Overview

"Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and ""tricks of the trade"" to both experienced researchers and more junior members in the lab."

Full Product Details

Author:   H. William Detrich III (Professor of Biochemistry and Marine Biology at Northeastern University) ,  Leonard I. Zon (Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA) ,  Monte Westerfield (Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Dimensions:   Width: 19.10cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.690kg
ISBN:  

9780123751607

ISBN 10:   0123751608
Pages:   400
Publication Date:   30 October 2009
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1. Haploid Screens and Gamma-Ray Mutagenesis Charline Walker 2. Positional Cloning of Mutated Zebrafish Genes Will Talbot 3. The Zebrafish Genome John Postlethwait 4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish Adam Amsterdam 5. Genetic Screens for Maternal-Effect Mutations Francisco Pelegri 6. Behavior Screening Assays in Zebrafish Herwig Baier 7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish Cecelia B. Moens 8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element Koichi Kawakami 9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish Charles Lee 10. The Zon Laboratory Guide to Positional Cloning in Zebrafish Leonard I. Zon 11. Sleeping Beauty Transposon for Efficient Gene Delivery Stephen C. Ecker 12. Transgene Manipulation in Zebrafish by Using Recombinases Gary W. Stuart 13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI Joachim Wittbrodt 14. Cloning Zebrafish by Nuclear Transfer Shuo Lin 15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening Christine Thisse 16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish Bill Trevarrow 17. Common Diseases of Laboratory Zebrafish Jennifer L. Matthews 18. Zebrafish Sperm Cryopreservation Thomas Look

Reviews

Author Information

"Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons ""on the ice"" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms."

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