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OverviewThe two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements.Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints. Full Product DetailsAuthor: Terry Brown (Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology)Publisher: Oxford University Press Imprint: Oxford University Press Edition: 2nd Revised edition Volume: 255 Dimensions: Width: 19.40cm , Height: 2.20cm , Length: 25.30cm Weight: 0.792kg ISBN: 9780199636457ISBN 10: 0199636451 Pages: 310 Publication Date: 15 November 2001 Audience: Professional and scholarly , Professional and scholarly , Professional & Vocational , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: Out of stock Table of Contents1: T A Brown: Strategies for research in molecular biology 2: Anna-Maria Frischauf: Construction of genomic libraries in lambda and cosmic vectors 3: Madan K Bhattacharyya: Construction of a cDNA library 4: C R Mundy, M W Cunningham and C A Read: Nucleic acid labelling and detection 5: N J Dyson: Immobilization of nucleic acids and hybridization analysis 6: T A Brown: DNA sequencing 7: T A Brown: The polymerase chain reaction 8: John M Ward: Protein expression in Escherichia coli 9: Colin P Smith: Transcript mapping Appendices 1: Health hazards and safety procedures 2: Equipment for molecular biology research 3: Important Escherichia coli strains 4: Recipes and general procedures 5: Restriction endonucleases 6: DNA and RNA modification enzymes 7: List of suppliers' addressesReviewsPractical information for working researchers in the life sciences interested in learning the basic principles of gene cloning and recombinant DNA techniques. The contributors provide the background material novices need to understand how a technique works, what it achieves, and how to make modifications to suit their own requirements, as well as advice on more advanced techniques. Vol. 2 describes procedures for isolating and studying individual genes, preparation and screening of libraries, polymerase chain reactions, DNA sequencing, and studying gene expression. The latest edition reflects the dramatic expansion in the range and sophistication of molecular biology techniques. --SciTech Book News<br> Practical information for working researchers in the life sciences interested in learning the basic principles of gene cloning and recombinant DNA techniques. The contributors provide the background material novices need to understand how a technique works, what it achieves, and how to make modifications to suit their own requirements, as well as advice on more advanced techniques. Vol. 2 describes procedures for isolating and studying individual genes, preparation and screening of libraries, polymerase chain reactions, DNA sequencing, and studying gene expression. The latest edition reflects the dramatic expansion in the range and sophistication of molecular biology techniques.--SciTech Book News Author InformationTab Content 6Author Website:Countries AvailableAll regions |