Essential Molecular Biology

Author:   Terry Brown (Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology)
Publisher:   Oxford University Press
Edition:   2nd Revised edition
Volume:   255
ISBN:  

9780199636457


Pages:   310
Publication Date:   15 November 2001
Format:   Hardback
Availability:   Out of stock   Availability explained


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Essential Molecular Biology


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Overview

The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements.Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.

Full Product Details

Author:   Terry Brown (Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Volume:   255
Dimensions:   Width: 19.40cm , Height: 2.20cm , Length: 25.30cm
Weight:   0.792kg
ISBN:  

9780199636457


ISBN 10:   0199636451
Pages:   310
Publication Date:   15 November 2001
Audience:   Professional and scholarly ,  Professional and scholarly ,  Professional & Vocational ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock   Availability explained

Table of Contents

1: T A Brown: Strategies for research in molecular biology 2: Anna-Maria Frischauf: Construction of genomic libraries in lambda and cosmic vectors 3: Madan K Bhattacharyya: Construction of a cDNA library 4: C R Mundy, M W Cunningham and C A Read: Nucleic acid labelling and detection 5: N J Dyson: Immobilization of nucleic acids and hybridization analysis 6: T A Brown: DNA sequencing 7: T A Brown: The polymerase chain reaction 8: John M Ward: Protein expression in Escherichia coli 9: Colin P Smith: Transcript mapping Appendices 1: Health hazards and safety procedures 2: Equipment for molecular biology research 3: Important Escherichia coli strains 4: Recipes and general procedures 5: Restriction endonucleases 6: DNA and RNA modification enzymes 7: List of suppliers' addresses

Reviews

Practical information for working researchers in the life sciences interested in learning the basic principles of gene cloning and recombinant DNA techniques. The contributors provide the background material novices need to understand how a technique works, what it achieves, and how to make modifications to suit their own requirements, as well as advice on more advanced techniques. Vol. 2 describes procedures for isolating and studying individual genes, preparation and screening of libraries, polymerase chain reactions, DNA sequencing, and studying gene expression. The latest edition reflects the dramatic expansion in the range and sophistication of molecular biology techniques. --SciTech Book News<br>


Practical information for working researchers in the life sciences interested in learning the basic principles of gene cloning and recombinant DNA techniques. The contributors provide the background material novices need to understand how a technique works, what it achieves, and how to make modifications to suit their own requirements, as well as advice on more advanced techniques. Vol. 2 describes procedures for isolating and studying individual genes, preparation and screening of libraries, polymerase chain reactions, DNA sequencing, and studying gene expression. The latest edition reflects the dramatic expansion in the range and sophistication of molecular biology techniques.--SciTech Book News


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