Overview

This volume provides readers with the latest technologies to study changes in the epitranscriptome. The protocols described in this book explore both targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and detection and quantification of pseudouridine in RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Epitranscriptomics: Methods and Protocols is an important resource for both expert and novice scientists who are interested in learning more aboutthis field.

Full Product Details

Author:   Narendra Wajapeyee ,  Romi Gupta
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1870
Weight:   1.036kg
ISBN:  

9781493988075

ISBN 10:   1493988077
Pages:   298
Publication Date:   12 December 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface...Table of Contents...Contributing Authors... 1. Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-MethylcytosineLukas Trixl, Dietmar Rieder, Thomas Amort, and Alexandra Lusser 2. Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular BeaconsMingming Chen, Shiqi Mao, Xiaotian Wu, Zhao Ma, Yantao Yang, Christopher J. Krueger, and Antony K. Chen 3. Visualization of Xist Long Non-Coding RNA with Fluorescent CRISPR/CAS9 SystemUrszula Wasko, Zeming Zheng, and Sanchita Bhatnagar 4. HAMR: High-Throughput Annotation of Modified RibonucleotidesLee E. Vandivier, Zachary D. Anderson, and Brian D. Gregory 5. High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-seq)Phillip J. Hsu and Chuan He 6. Direct Chemical Biotinylation of RNA 5'-Ends Using a Diazo ReagentGreggory M. Rice, Razvan Nutiu, and Christian M. Gampe 7. Identification of Methylated Transcripts Using the TRIBE ApproachLina Worpenberg, Tobias Jakobi, Christoph Dieterich, and Jean-Yves Roignant 8. Decoding the Atlas of RNA Modifications from Epitranscriptome Sequencing DataXiao-qin Zhang and Jian-hua Yang 9. Detection of MicroRNA-Mediated Target mRNA Cleavage and 3'-Uridylation in Human Cells by a SLA-RT-PCR AnalysisJing Lin and Lin Ji 10. Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer PipelineRui Dong, Xu-Kai Ma, Ling-Ling Chen, and Li Yang 11. Synthesis and Evaluation of Novel Neamine-Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S Ribosomal RNAZhen-Jun Yang, Maria-Grazia Concilio, Vasudevan Ramesh, and Li-He Zhang 12. Electron Microscope Detection of 5-Methylcytosine on DNA and RNAIrene Masiello and Marco Biggiogera 13. Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified NucleosidesEikan Mishima and Takaaki Abe 14. LncVar:Deciphering Genetic Variations Associated with Long Non-Coding GenesXiaowei Chen, Yajing Hao, Ya Cui, Zhen Fan, and Runsheng Chen 15. Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANESandro Andreotti and Stefan Canzar 16. Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNAGianluigi Lichinchi and Tariq M. Rana 17. Detection and Quantification of Pseudouridine in RNAHironori Adachi, Meemanage D. DeZoysa, and Yi-Tao Yu 18. 5-Methylcytosine Analysis by RNA-BisSeqYu-Sheng Chen, Hai-Li Ma, Ying Yang, Wei-Yi Lai, Bao-Fa Sun, and Yun-Gui Yang 19. RNA Modification Regulatory Genes in DNA DamageRadoslav Janostiak and Narendra Wajapeyee 20. Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNAArvindhan Nagarajan, Radoslav Janostiak, and Narendra Wajapeyee

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