Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols

Author:   Nicole I. Nieden
Publisher:   Humana Press Inc.
Edition:   2011
Volume:   690
ISBN:  

9781607619611


Pages:   336
Publication Date:   02 November 2010
Format:   Hardback
Availability:   In Print   Availability explained
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Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols


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Full Product Details

Author:   Nicole I. Nieden
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2011
Volume:   690
Dimensions:   Width: 17.80cm , Height: 2.50cm , Length: 25.40cm
Weight:   0.852kg
ISBN:  

9781607619611


ISBN 10:   160761961
Pages:   336
Publication Date:   02 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Embryonic Stem Cells for Osteo-Degenerative Diseases.- Methods for Culturing Mouse and Human Embryonic Stem Cells.- Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells.- Functional Assays for Human Embryonic Stem Cell Pluripotency.- Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells.- Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters.- Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives.- Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers.- Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology.- Methods for Embryoid Body Formation: The Microwell Approach.- Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview.- Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells.- Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the “Raclure” Method.- Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes.- Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds.- Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells.- Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield.- Identification of Osteoclasts in Culture.- Analysis of Glycosaminoglycans in Stem Cell Glycomics.- Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent.- Measurement and Illustration of Immune Interaction after Stem Cell Transplantation.

Reviews

From the reviews: Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ... This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ... The authors provide excellent minireviews and great easy-to-follow research protocols. ... The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases. (Bruce A. Fenderson, Doody's Review Service, June, 2011)


From the reviews: Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ! This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ! The authors provide excellent minireviews and great easy-to-follow research protocols. ! The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases. (Bruce A. Fenderson, Doody's Review Service, June, 2011)


From the reviews: Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ... This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ... The authors provide excellent minireviews and great easy-to-follow research protocols. ... The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases. (Bruce A. Fenderson, Doody's Review Service, June, 2011)


From the reviews: Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. ... This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. ... The authors provide excellent minireviews and great easy-to-follow research protocols. ... The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases. (Bruce A. Fenderson, Doody's Review Service, June, 2011)


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