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OverviewBarley represents one of the economically most important and widely distributed crops worldwide, and genetic engineering is expected to play a crucial role in its further improvement. However, once transgenic plants are obtained, the selectable marker gene is not necessary anymore or even unwanted, and thus viable means to circumvent the use of selectable markers or to remove them from transgenic plants are required. In the present study, a novel strategy has been pursued to generate selectable marker-free transgenic lines instantly homozygous for the gene-of-interest. Primary co-transgenic plants (T0) containing both the selectable marker gene (hygromycin phosphotransferase) and a model gene-of-interest (ß-glucuronidase) were produced via Agrobacterium-mediated gene transfer using two independent T-DNAs. Both removal of the selectable marker and establishment of instant homozygosity of the gene-of-interest was then achieved at a time through segregation of uncoupled T-DNAs in populations of doubled haploid (DH) lines produced from the T0 plants via embryogenic pollen culture. Full Product DetailsAuthor: Eszter KapusiPublisher: Sudwestdeutscher Verlag Fur Hochschulschriften AG Imprint: Sudwestdeutscher Verlag Fur Hochschulschriften AG Dimensions: Width: 15.20cm , Height: 0.80cm , Length: 22.90cm Weight: 0.200kg ISBN: 9783838135656ISBN 10: 3838135652 Pages: 128 Publication Date: 21 December 2012 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |