Overview

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls  Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Full Product Details

Author:   Christian Dahmann
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2022
Volume:   2540
Weight:   1.012kg
ISBN:  

9781071625408

ISBN 10:   1071625403
Pages:   424
Publication Date:   19 August 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

                        1.              Using FlyBase, a database of Drosophila genes and genetics Victoria K. Jenkins, Aoife Larkin, Jim Thurmond and The FlyBase Consortium 2.              The Q-System: A versatile repressible binary expression system Orsolya Fölsz, Chun-Chieh Lin, Darya Task, Olena Riabinina and Christopher J. Potter 3.              Resources and methods for the analysis of microRNA function in Drosophila Sromana Mukherjee and Nicholas Sokol 4.              Analysis of single-cell transcriptome data in Drosophila Schayan Yousefian, Maria Jelena Musillo and Josephine Bageritz                                5.              Prime editing for precise genome engineering in Drosophila Justin A. Bosch and Norbert Perrimon 6.              CRISPR/Cas9 mediated precise and efficient genome editing in Drosophila Kevin G. Nyberg and Richard W. Carthew 7.              Tissue-specific CRISPR-Cas9 screening in Drosophila Fillip Port and Michael Boutros 8.              CRISPR-based transcriptional activation in Drosophila Yuting Han, Xinyi Lu, Yutong Li, Yuhao Qiu, Xizhi Dong, Xiaochen Li, Xu Si, Qingfei Liu, Jian-Quan Ni 9.              Tracing and manipulating Drosophila cell lineages based on CRISPR: CaSSA and CLADES Jorge Garcia-Marques and Tzumin Lee 10.           Studying protein function using nanobodies and other protein binders in Drosophila Katarzyna Lepeta, Milena Bauer, Gustavo Aguilar, Alessandra Vigano, Shinya Matsuda and Markus Affolter 11.           Anchor Away: A system for fast inhibition of proteins in Drosophila Pablo Sanchez Bosch 12.           Tagging Drosophila proteins with genetically-encoded fluorophores Jerome Avellaneda and Frank Schnorrer 13.           Optogenetic methods to control tissue mechanics in Drosophila Daniel Krueger and Stefano De Renzis 14.           Optochemical control of cell contractility in Drosophila embryos Deqing Kong and Jörg Großhans 15.           Stiffness measurement of Drosophila egg chambers by atomic force microscopy Uwe Töpfer, Karla Yanín Guerra Santillán and Elisabeth Fischer-Friedrich 16.           Cultivation and live imaging of Drosophila imaginal discs Natalie A. Dye 17.           Sample preparation and imaging of the pupal Drosophila abdominal epidermis  Daiki Umetsu 18.           FlyClear: A tissue clearing technique for high-resolution microscopy of Drosophila Marko Pende, Saiedeh Saghafi, Klaus Becker, Thomas Hummel and Hans-Ulrich Dodt 19.           Preparation of Drosophila tissues and organs for transmission electron microscopy Olympia-Ekaterini Psathaki and Achim Paululat 20.           Segmentation and quantitative analysis of epithelial tissues Benoit Aigouy and Benjamin Prud’homme 21.           Genetically-encoded sensors to study metabolism in Drosophila Ellen McMullen, Helen Hertenstein, Stephan Müller and Stefanie Schirmeier

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