Overview

DNA transfer to cultured cells Edited by Katya Ravid and R. Ian Freshney Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics. DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including: * Viral infection * Electroporation * Phosphate precipitation * DEAE Dextran * Liposomes * Yeast artificial chromosomes (YACs) * Whole chromosome transfer * Enhanced expression. Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.

Full Product Details

Author:   Katya Ravid (Boston University Medical Center, Boston, Massachusetts) ,  R. Ian Freshney (University of Glasgow, Scotland)
Publisher:   John Wiley & Sons Inc
Imprint:   Wiley-Liss Inc.,U.S.
Dimensions:   Width: 18.00cm , Height: 1.40cm , Length: 25.40cm
Weight:   0.544kg
ISBN:  

9780471165729

ISBN 10:   0471165727
Pages:   312
Publication Date:   21 August 1998
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

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Reviews

..a very useful book. (Cell Biology International, Vol 24/7, 2000)

Author Information

Katya Ravid is the editor of DNA Transfer to Cultured Cells, published by Wiley. R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

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