Overview

Well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.

Full Product Details

Author:   Pat Vaughan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   152
Dimensions:   Width: 15.20cm , Height: 1.40cm , Length: 22.90cm
Weight:   1.090kg
ISBN:  

9780896036437

ISBN 10:   089603643
Pages:   192
Publication Date:   07 July 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Assays To Detect And Quantitate DNA Repair Activity.- Repair of A/G and A/8-oxoG Mismatches by MutY Adenine DNA Glycosylase.- Assays for the Repair of Oxidative Damage by Formamidopyrimidine Glycosylase (Fpg) and 8-)Oxoguanine DNA Glycosylase (OGG-1).- Detection and Quantitation of Uracil DNA Glycosylase Activity.- Detection of DNA Deoxyribophosphodiesterase Activity.- O 6-Alkylguanine-DNA Alkytransferase Assay.- The E. coli Vsr Endonuclease.- Coupling of DNA Helicase Function to DNA Strand Exchange Activity.- Detection and Quantitation of RecBCD Enzyme (Exonuclease V) Activity.- Holliday Junction Branch Migration and Resolution.- MutS–DNA Interactions and DNase Protection Analysis with Surface Plasmon Resonance.- Use Of DNA Repair Enzymes As Tools In Molecular Biology.- Flexible Genetic Engineering Using RecA Protein.- Interspecies Recombination and Mismatch Repair.- The Use of Immobilized Mismatch Binding Protein in Mutation/SNP Detection.- Use of Uracil DNA Glycosylase in the Detection of Known DNA Mutations and Polymorphisms.- Use of Uracil DNA Glycosylase in Scanning for Unknown DNA Mutations and Polymorphisms.- The Use of Resolvases T4 Endonuclease VII and T7 Endonuclease I in Mutation Detection.

Reviews

must for any science library supporting a molecular biology program. - E-Streams An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher. - Lavoisier Librairie

...must for any science library supporting a molecular biology program. - E-Streams An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher. - Lavoisier Librairie

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