Overview

The first edition of this book, published in 1999 and called DNA Repair Protocols: Eukaryotic Systems, brought together laboratory-based methods for studying DNA damage and repair in diverse eukaryotes: namely, two kinds of yeast, a nematode, a fruit fly, a toad, three different plants, and human and murine cells. This second edition of DNA Repair Protocols covers mammalian cells only and hence its new subtitle, Mammalian Systems. There are two reasons for this fresh emphasis, both of them pragmatic: to cater to the interests of what is now a largely mammalocentric DNA repair field, and to expedite editing and prod- tion of this volume. Although DNA Repair Protocols: Mammalian Systems is a smaller book than its predecessor, it actually contains a greater variety of methods. Fourteen of the book’s thirty-two chapters are entirely new and areas of redundancy present in the first edition have been eliminated here (for example, now just two chapters describe assays for nucleotide excision repair [NER], rather than seven). All eighteen returning chapters have been revised, many of them ext- sively. In order to maintain a coherent arrangement of topics, the four-part p- titioning seen in the first edition was dispensed with and chapters concerned with ionizing radiation damage and DNA strand breakage and repair were re- cated to near the front of the book. Finally, an abstract now heads each chapter.

Full Product Details

Author:   Daryl S. Henderson
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd Revised edition
Volume:   314
Dimensions:   Width: 15.50cm , Height: 2.80cm , Length: 23.50cm
Weight:   1.980kg
ISBN:  

9781588295132

ISBN 10:   1588295133
Pages:   498
Publication Date:   01 October 2005
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
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Table of Contents

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating.- Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes.- Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities.- Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation.- Inhibition of DNA Synthesis by Ionizing Radiation.- Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication.- Cytometric Assessment of Histone H2AX Phosphorylation.- Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication).- In Vitro Rejoining of Double-Strand Breaks in Genomic DNA.- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR.- Plasmid-Based Assays for DNA End-Joining In Vitro.- Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants.- Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage.- Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells.- Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR.- Immunochemical Detection of UV-Induced DNA Damage and Repair.- A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts.- Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay.- DNA Damage Quantitation by Alkaline Gel Electrophoresis.- The Comet Assay.- Fast Micromethod DNA Single-Strand-Break Assay.- 32P-Postlabeling DNA Damage Assays.- Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA.- Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision.- Characterization of Enzymes ThatInitiate Base Excision Repair at Abasic Sites.- Base Excision Repair in Mammalian Cells.- In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts.- Biochemical Assays for the Characterization of DNA Helicases.- Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA.- Assaying for the Dual Incisions of Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site.- Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated With DNA Excision Repair Sites in Mammalian Cells.- Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates.

Reviews

.,. It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches. - Anticancer Research <br>.,. must for any science library supporting a molecular biology program. - E-Streams <br> An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher. - Lavoisier Librairie <p>.,. an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair. Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts. -Int. J. Radiation Oncology Biol. Phys. <p> This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety. -BTS Newsletter

...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches. - Anticancer Research ...must for any science library supporting a molecular biology program. - E-Streams An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher. - Lavoisier Librairie ...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair. Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts. -Int. J. Radiation Oncology Biol. Phys. This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety. -BTS Newsletter

"""...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches."" - Anticancer Research ""...must for any science library supporting a molecular biology program."" - E-Streams ""An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher."" - Lavoisier Librairie ""...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair.  Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Protocols: Mammalian Systems succeeds on both counts."" -Int. J. Radiation Oncology Biol. Phys. ""This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety."" -BTS Newsletter"

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