Overview

Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions and define their components, their manner of interaction, and their manner of function, both in the cell and in the test tube. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to understand DNA-protein interactions.

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9780896036710

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The topics are organized in a sensible linear manner, starting with elementary protein-DNA binding assays such as filter binding and EMSA, leading to footprinting techniques for the identification of individual contacts and DNA distortion, through more complex crosslinking and spectroscopic methods, to the final chapters on crystallization and some highly specialized biophysical techniques such as force microscopy....this book will find favor with both the novice and the expert; the former will learn techniques that will be useful for many years whilst the latter will be inspired by discovering new applications. - Molecular Biotechnology

The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual. - Quarterly Review of Biology

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