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OverviewDNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins. Full Product DetailsAuthor: Ken Mills , BH Ramsahoye (Humana author USA)Publisher: Humana Press Inc. Imprint: Humana Press Inc. Volume: v. 200 Dimensions: Width: 15.20cm , Height: 1.80cm , Length: 22.90cm Weight: 0.446kg ISBN: 9780896036185ISBN 10: 0896036189 Pages: 176 Publication Date: 01 March 2002 Audience: College/higher education , Professional and scholarly , Undergraduate , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: Out of stock  Table of ContentsOverview. Nearest-Neighbor Analysis. Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography. Methylation Analysis by Chemical DNA Sequencing. Methylation-Sensitive Restriction Fingerprinting. Restriction Landmark Genome Scanning. Combined Bisulfite Restriction Analysis (COBRA). Differential Methylation Hybridization Using CpG Island Arrays. Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences. Isolation of CpG Islands Using a Methyl-CpG Binding Column. Purification of MeCP2-Containing Deacetylase from Xenopus laevis. DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method. Measuring DNA Demethylase Activity In Vitro. Extracting DNA Demethylase Activity from Mammalian Cells. Index.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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