Overview

This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNA-Encoded Chemical Libraries:Methods and Protocols is a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.

Full Product Details

Author:   David Israel ,  Yun Ding
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2541
Weight:   0.649kg
ISBN:  

9781071625446

ISBN 10:   1071625446
Pages:   226
Publication Date:   10 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preface… Table of Contents… Contributing Authors… 1. Library Synthesis: Building Block Selection, Handling, and Tracking Katelyn J. Billings and Melissa C. Grenier-Davies 2. Library Synthesis: Building Block Validation Melissa C. Grenier-Davies 3. Library Synthesis and Analytics Jing Chai 4. Chemical Ligation of Oligonucleotide Tags to Support Encoded Chemical Library Synthesis Alexander Litovchick and Anthony D. Keefe 5. On-DNA Reductive Amination and Alkylation Jing Chai 6. On-DNA-1,2,3-Triazole Formation via Click Reaction Sarah K. Scott 7. On-DNA Suzuki-Miyaura Cross-Coupling Yun Ding 8. On-DNA Pd and Cu Promoted C-N Cross-Coupling Reactions Xiaojie Lu and Xuan Wang 9. Palladium Promoted On-DNA Heck Reactions Xuan Wang and Xiaojie Lu 10. Ruthenium Promoted On-DNA C-H Activation Reaction Xuan Wang and Xiaojie Lu 11. DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles Huang-Chi Du, Ying-Chu Chen, and Hongbing Huang 12. Condensation of DNA-Conjugated Imines with Homophthalic Anhydride for the Synthesis of Isoquinolones On-DNA Josephine Yuen, Jing Chai, and Yun Ding 13. DNA Encoding of Natural Products Hongtao Xu and Peixiang Ma 14. Initiating DNA-Encoded Library Synthesis with a Hexathymidine DNA Oligonucleotide Mateja Klika Škopić, Denise dos Santos Smith, Anne Gohla, Verena B. K. Kunig, and Andreas Brunschweiger 15. One Bead-One Compound (OBOC) Peptidomimetic Encoded Library Synthesis via Split-and-Pool Methods Claudio Aquino and Mohosin Sarkar 16. DNA Conjugates as Tool Compounds for DEL Selections David I. Israel 17. Quantitation of DNA Encoded Libraries by qPCR Christopher S. Kollmann 18. Target Protein Design and Pre-Selection Analysis Qiuxia Chen and David I. Israel 19. DEL Selections Against a Soluble Protein Target Qiuxia Chen and Junyang Zhu 20. Selection Method of DNA-Encoded Chemical Library for Irreversible Covalent Binders Zhengrong Zhu 21. Next Generation Sequencing of DNA-Encoded Libraries Jian Liu and You Li 22. Translation of DNA Sequence to Chemical Structure in DNA Encoded Libraries You Li 23. Analysis of DNA-Encoded Library Screening Data: Selection of Molecules for Synthesis Alexander L. Satz and Weiren Cui 24. High Throughput Binder Confirmation (HTBC) Bing Xia and Jennifer D. Summerfield 25. High-Throughput Binder Confirmation Using Affinity Selection Mass Spectrometry Eric X. Shi Subject Index List…

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