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OverviewThis volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. Full Product DetailsAuthor: Nima MosammaparastPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 1st ed. 2022 Volume: 2444 Weight: 0.765kg ISBN: 9781071620625ISBN 10: 1071620622 Pages: 284 Publication Date: 16 March 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1.Robust Computational Approaches to Defining Insights on the Interface of DNA Repair with Replication and Transcription in Cancer Albino Bacolla and John A. Tainer 2. A Whole Genome CRISPR/Cas9 Screening Approach for Identifying Genes Encoding DNA End-Processing Proteins Bo-Ruei Chen and Barry P. Sleckman 3.Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells Brittany A. Townley, Jennifer M. Soll, and Nima Mosammaparast 4.Universally Accessible Structural Data on Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray Scattering for DNA Repair Insights Naga Babu Chinnam, Aleem Syed, Kathryn Burnett, Greg Hura, John A. Tainer, and Susan E. Tsutakawa 5.Assessing DNA damage responses using B lymphocyte cultures. Rachel Johnston, Lynn S. White, and Jeffrey J. Bednarski 6.Studying single stranded DNA gaps at replication intermediates by electron microscopy Jessica Jackson and Alessandro Vindigni 7.Approaches to monitor termination of DNA replication using Xenopus egg extracts Tamar Kavlashvili and James M. Dewar 8.Use of high-performance liquid chromatography coupled to mass spectrometry to quantify modified nucleosides Rebecca Rodell, Adit Ganguly, Ning Tsao, and Nima Mosammaparast 9. Targeted Formation of 8-oxoguanine in Telomeres Ryan P. Barnes, Sanjana A. Thosar, Elise Fouquerel, and Patricia L. Opresko 10. Qualitative and Quantitative Analysis of DNA-Cytidine Deaminase Activity Rachel DeWeerd and Abby M. Green 11. Characterization of DNA-PK-bound end fragments using GLASS-ChIP Rajashree A. Deshpande and Tanya T. Paull 12. Monitoring Nuclease Activity by X-ray Scattering Interferometry using Gold Nanoparticle Conjugated DNA Daniel J. Rosenberg, Aleem Syed, John A. Tainer, and Greg L. Hura 13. In Vitro Reconstitution of BRCA1-BARD1/RAD51 Mediated Homologous DNA Pairing Meiling Wang, Cody M. Rogers, Dauren Alimbetov, and Weixing Zhao 14. Purification of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) from HeLa cells Linda Lee, Yaping Yu, and Susan P. Lees-Miller 15. Purification and characterization of human DNA ligase III complexes after expression in insect cells Ishtiaque Rashid, Miaw-Sheue Tsai, Aleksandr Sverzhinsky, Aye Su Hlaing, Brian Shih, Aye C. Thwin, Judy G. Lin, Su S. Maw, John M. Pascal, and Alan E. Tomkinson 16. Generation of monoubiquitin and K63-linked polyubiquitin chains for protein interaction studies Rita Anoh, Kate A. Burke, Dhane P. Schmelyun, and Patrick M. LombardiReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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