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OverviewThis volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories. It covers transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organisation and expression, in vitro mutagenesis, and DNA sequencing. The information provided is a completely up to date account of each topic by established researchers. These two volumes, the first of four, are a thoroughly revised and up dated version of the original ones published in the Practial Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories. Full Product DetailsAuthor: D. M. Glover (Professor, Department of Anatomy and Physiology, Professor, Department of Anatomy and Physiology, University of Dundee) , B. D. Hames (Doctor, Department of Biochemistry and Molecular Biology, Doctor, Department of Biochemistry and Molecular Biology, University of Leeds)Publisher: Oxford University Press Imprint: Oxford University Press Edition: 2nd Revised edition Volume: 148 Dimensions: Width: 15.70cm , Height: 1.60cm , Length: 23.50cm Weight: 0.499kg ISBN: 9780199634767ISBN 10: 0199634769 Pages: 288 Publication Date: 11 May 1995 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: To order  Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of Contents1. Techniques for transformation of E.coli ; 2. Lambda replacement vectors in the construction of genomic DNA libraries ; 3. Procedures for cDNA cloning ; 4. Making probes ; 5. Using cloned DNAs to analyse gene organization and expression ; 6. Choices of single-stranded vectors and their application for in vitro mutagenesis ; 7. PCR and its applications ; 8. DNA sequencingReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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