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OverviewExpression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecularbiology laboratories. Full Product DetailsAuthor: D. M. Glover (Professor, Department of Anatomy and Physiology, Professor, Department of Anatomy and Physiology, University of Dundee) , B. D. Hames (Department of Biochemistry and Molecular Biology, Department of Biochemistry and Molecular Biology, University of Leeds)Publisher: Oxford University Press Imprint: Oxford University Press Edition: 2nd Revised edition Volume: 149 Dimensions: Width: 15.70cm , Height: 1.50cm , Length: 23.50cm Weight: 0.465kg ISBN: 9780199634781ISBN 10: 0199634785 Pages: 274 Publication Date: 11 May 1995 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: To order  Stock availability from the supplier is unknown. We will order it for you and ship this item to you once it is received by us. Table of Contents1. Screening expression libraries with antibody and protein probes ; 2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies ; 3. Purification of over-produced proteins from E.coli cells ; 4. Production of monoclonal antibodies against proteins expressed in E.coli ; 5. Expression of cloned genes in yeast ; 6. Intraction trap cloning with yeast ; 7. The baculovirus expression systemReviewsa worthwhile bench top companion Henry Brzeski, University of Strathclyde, Biochemical Systematics and Ecology, Vol. 24, No. 1, 1996 The present book consists of 7 chapters that each coprehensively describe different aspects of the subject and which may be read independently ... this book is very detailed and informative and contains much information with key literature citations of use for any scientist working in the area of heterologous protein expression ... The book can thus be highly recommended. Bent Honore, FEBS Letters 393 (1996) `a worthwhile bench top companion' Henry Brzeski, University of Strathclyde, Biochemical Systematics and Ecology, Vol. 24, No. 1, 1996 `The present book consists of 7 chapters that each coprehensively describe different aspects of the subject and which may be read independently ... this book is very detailed and informative and contains much information with key literature citations of use for any scientist working in the area of heterologous protein expression ... The book can thus be highly recommended.' Bent Honoré, FEBS Letters 393 (1996) Author InformationTab Content 6Author Website:Countries AvailableAll regions |
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