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OverviewThis thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others. Full Product DetailsAuthor: Akash GautamPublisher: Springer Nature Switzerland AG Imprint: Springer Nature Switzerland AG Edition: 1st ed. 2022 Weight: 0.500kg ISBN: 9783030942298ISBN 10: 3030942295 Pages: 203 Publication Date: 30 March 2022 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of Contents1-Basic idea about nucleic acids for non-experts 2-General steps during isolation of DNA and RNA 3-Phenol-chloroform DNA isolation method 4-RNA isolation by the guanidinium-acid-phenol method 5-Spin column-based isolation of nucleic acid 6-Isolation of plasmid DNA by alkaline lysis 7-DNA Isolation by Hydrophilic Ionic Liquid Treatment 8-Lithium Chloride based isolation of DNA and RNA 9-Isolation of Bacteriophage DNA by PEG Method 10-DNA isolation by Chelex Method 11-DNA isolation by Lysozyme and Proteinase K 12-Salting-out method for blood samples 13-CTAB or SDS based isolation of plant’s DNA 14-DNA extraction by spooling method 15-Magnetic bead-based nucleic acid isolation 16-Density gradient-based nucleic acid isolation 17-DNA extraction from agarose gel though paper-strips 18-TRANSFORMATION OR GENETIC MODIFICATION OF CELLS/ORGANISMS 19-GENE CLONING AND VECTORS 20-POLYMERASE CHAIN REACTION (PCR) 21-Southern and Northern Blotting 22-GENOME MAPPING 23-Applications of DNA Sequencing Technologies for Current ResearchReviewsAuthor InformationDr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020). Tab Content 6Author Website:Countries AvailableAll regions |
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