Overview

The second edition of the highly acclaimed Differential Display Methods and Protocols consists of a wider-ranging collection of chapters that highlight both recent methodological refinements and some fine examples of differential display (DD) applications in recent years. Part I covers gene discovery methodologies that have a unique place in the modern molecular biological toolbox for gene expression analysis while Part II demonstrates the power of DD technology by showcasing select applications that have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription re3pression, gene silencing, mRNA polymorphism and protein-RNA interaction.

Full Product Details

Author:   Peng Liang ,  Jonathan Meade ,  Arthur B. Pardee
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2nd ed. 2006
Volume:   317
Dimensions:   Width: 15.50cm , Height: 2.70cm , Length: 23.50cm
Weight:   0.732kg
ISBN:  

9781588293381

ISBN 10:   1588293386
Pages:   320
Publication Date:   21 October 2005
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Methodologies.- Global Analysis of Gene Expression by Differential Display.- Automation of Fluorescent Differential Display With Digital Readout.- Ordered Differential Display.- GeneCalling®.- High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR.- Vertical Arrays.- Automated Pattern Ranking in Differential Display Data Analysis.- Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs.- Applications.- Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma.- Identification of Disease Markers by Differential Display.- Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display.- Identification of p53-Regulated Genes by the Method of Differential Display.- Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes.- Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display.- Functional Analysis of Nocturnin.- Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display.- Identification of Target Genes of a Yeast Transcriptional Repressor.- Detection of an mRNA Polymorphism by Differential Display.- Silencing in Yeast.- Identification of mRNA Bound to RNA Binding Proteins by Differential Display.

Reviews

The variety of applications...is so broad that almost every newcomer...can use these protocols as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field. -Trends in Biotechnology <br> From Reviews of the First Edition...<br> .,. really helpful for all geneticists and cellular and molecular biologists...a precious book which should be in all hands! -Cellular and Molecular Biology <br> .,. For any would-be gene hunter thinking of or already practicing differential display, this book will provide an invaluable and long overdue guide to the technique. -Molecular Medicine <br> .,. .an ideal resource for any research group interested in using DD. -Microbiology Today <br> .,. highly recommend[ed]...for purchase by everyone interested in the comparative gene expression, , and especially to newcomers to the field. -Trends in Biotechnology<br>

The variety of applications...is so broad that almost every newcomer...can use these protocols as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field. -Trends in Biotechnology From Reviews of the First Edition... ...really helpful for all geneticists and cellular and molecular biologists...a precious book which should be in all hands! -Cellular and Molecular Biology ...For any would-be gene hunter thinking of or already practicing differential display, this book will provide an invaluable and long overdue guide to the technique. -Molecular Medicine ...an ideal resource for any research group interested in using DD. -Microbiology Today ...highly recommend[ed]...for purchase by everyone interested in the comparative gene expression,, and especially to newcomers to the field. -Trends in Biotechnology

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