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OverviewDifferential Display explains in detail how to perform the technique of RT-PCR Differential display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniques and automatic image analysis. Full Product DetailsAuthor: Ron A. Leslie (SmithKline Beecham Pharmaceuticals, Harlow) , Harry A. Robertson (Dalhousie University, Halifax)Publisher: Oxford University Press Imprint: Oxford University Press Volume: 224 Dimensions: Width: 18.90cm , Height: 1.70cm , Length: 24.70cm Weight: 0.575kg ISBN: 9780199637584ISBN 10: 019963758 Pages: 274 Publication Date: 04 May 2000 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Out of Print Availability: Out of stock  Table of ContentsAn introduction to differential display and related techniques Recent advances in fluorescent differential display Practical aspects of the experimental design for differential display of transcripts obtained from complex tissues RAP-array : expression profiling using reduced complexity probes for cDNA arrays The use of RT-PCR differential display in single-celled organisms and plant tissues A modified approach for the efficient display of 3' - end restriction fragments of cDNAs Cloning of differentially expressed brain cDNAs Use of suppression subtractive hybridization strategy to identify differential gene expression in brain ischemic tolerance Studying gene expression profiles in specialized brain regions by microsage Construction and applications of gene microarrays on nylon membranes Subtracted differential display and antisense approaches to analyzing brain signalling Differential display analysis of memory-associated genesReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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