Overview

Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.

Full Product Details

Author:   Rocky S. Tuan ,  Cecilia W. Lo
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   136
Dimensions:   Width: 17.00cm , Height: 3.20cm , Length: 24.40cm
Weight:   1.085kg
ISBN:  

9780896035751

ISBN 10:   0896035751
Pages:   505
Publication Date:   17 January 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Developmental Biology Protocols.- Organogenesis.- Drosophila as a Genetic Tool to Define Vertebrate Pathway Players.- Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis.- Analysis of Mammary Gland Morphogenesis.- Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro.- Craniofacial Development and Patterning.- Craniofacial Skeletel Morphogenesis In Vitro.- Skeletal Morphogenesis.- Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration.- Retroviral Infection of T-Cell Precursors in Thymic Organ Culture.- Assay for the Isolation of Hepatogenic Factors.- Skin Morphogenesis.- Apoptosis in Development.- Methods to Detect Patterns of Cell Death in Drosophila.- Abnormal Development and Teratology.- Mechanisms of Neurulation.- Neurulation and Neural Tube Closure Defects.- Neural Tube Defects.- Experimental Manipulation and Morphometric Analysis of Neural Tube Development.- Isolation of Neuroepithelium and Formation of Minispheres.- Examination of Normal and Abnormal Placentation in the Mouse.- Palatal Dysmorphogenesis.- Palatal Dysmorphogenesis.- Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities.- Assessment of Laterality Defects in Rodent Embryos.- Cardiac Morphogenesis and Dysmorphogenesis I. Normal Development.- Analysis of Two Aspects of Left-Right Patterning of the Vertebrate Heart.- Biologically Based Risk Assessment Models for Developmental Toxicity.- Screening and Mapping of Novel Genes and Mutations.- Positional Cloning.- Gene Trapping in Embryonic Stem Cells In Vitro to Identify Novel Developmentally Regulated Genes in the Mouse.- PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes.- Analysis of mRNA ExpressionDuring Preimplantation Development.- Differential Screens with Subtracted PCR-Generated cDNA Libraries from Subregions of Single Mouse Embryos.- HPLC-Based mRNA Differential Display.- Production of Transgenic Drosophila.- Transgenic Manipulation of the Sea Urchin Embryo.- Transgenic Zebrafish.- Production of Avian Chimeras and Germline Transmission.- Incorporation of Genetically Modified Cells in Chicken Chimeras.- Long-Term Culture of Chicken Blastodermal Cells (CBCs) and Selection of Transfected CBCs Using Antibiotic Resistance.- Nuclear Transplantation and Cloning in Mammals.- Production of Transgenic Mice.- Production of Transgenic Mice with Yeast Artificial Chromosomes.- Gene-Targeting Strategies.- Chimeric Animals and GermlineTransmission.- Conditional Gene Knockout Using Cre Recombinase.- Application of Cre/loxP in Drosophila.

Reviews

We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This is the collection to get before your next grant application is due. - Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done! Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail...these protocols will be an invaluable aid to every geneticist and developmental biologist. - Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany

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