Overview

Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.

Full Product Details

Author:   Radhakrishnan Padmanabhan ,  Subhash G. Vasudevan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2014
Volume:   1138
Weight:   7.844kg
ISBN:  

9781493943715

ISBN 10:   1493943715
Pages:   403
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Dengue Virus Growth, Purification and Fluorescent Labeling.- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique.- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique.- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy.- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion.- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection.- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging.- Development and Application of Dengue Virus Reverse Genetic Systems.- DENV4 Renilla Luciferase Reporter Replicons.- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination.- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display.- Next Generation Whole Genome Sequencing of Dengue Virus.- MPGAfold in Dengue Secondary Structure Prediction.- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE.- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5.- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry.- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore.- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation.- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import.- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening.- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors.- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase.- Animal Models in Dengue.- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions