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OverviewThis second edition provides new and updated chapters from expert researchers in the field detailing methods used to study the multi-facet deep sequencing data field. Chapters guide readers through techniques for processing RNA-seq data, microbiome analysis, deep learning methodologies, and various approaches for the identification of sequence variants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deep Sequencing Data Analysis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field. Full Product DetailsAuthor: Noam ShomronPublisher: Springer-Verlag New York Inc. Imprint: Springer-Verlag New York Inc. Edition: 2nd ed. 2021 Volume: 2243 Weight: 0.728kg ISBN: 9781071611050ISBN 10: 1071611054 Pages: 374 Publication Date: 20 February 2022 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsDetecting Causal Variants in Mendelian Disorders using Whole Genome Sequencing.- Statistical Considerations on NGS Data for Inferring Copy Number Variations.- Applications of Community Detection Algorithms to Large Biological Datasets.- Processing and Analysis of RNA-seq data from Public Resources.- Improved Analysis of High-throughput Sequencing Data Using Small Universal k-mer Hitting Sets.- An Introduction to Whole-metagenome Shotgun Sequencing Studies.- Microbiome Analysis using 16S Amplicon Sequencing: From Samples to ASVs.- RNA-Seq in Non-model Organisms.- Deep Learning Applied on Next Generation Sequencing Data Analysis.- Interrogating the Accessible Chromatin Landscape of Eukaryote Genomes using ATAC-seq.- Genome-Wide Noninvasive Prenatal Diagnosis of SNPs and Indels.- Genome-wide Noninvasive Prenatal Diagnosis of De Novo Mutations.- Accurate Imputation of Untyped Variants from Deep Sequencing Data.- Multi-region Sequence Analysisto Predict Intratumor Heterogeneity and Clonal Evolution.- Overcoming Interpretability in Deep Learning Cancer Classification.- Single-cell Transcriptome Profiling.- Biological Perspectives of RNA-sequencing Experimental Design.- Analysis of microRNA Regulation in Single Cells.- DNA Data Collection and Analysis in the Forensic Arena.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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