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OverviewDeveloping targeted genome regulation approaches holds much promise for accelerating trait discovery and development in agricultural biotechnology. The type II CRISPR/Cas system has been adapted for genome editing and transcriptional modulation purposes across a variety of organisms. Here, we employed this system for targeted transcriptional regulation in planta by developing chimeric dCas9-based activators and repressors. By fusing activator and repressor domains to the C-terminus of dCas9, respectively, we generated transcriptional activators: dCas9.EDLL and dCas9.TAD, as well as transcriptional repressor: dCas9.SRDX. Our data demonstrate that the dCas9.EDLL and dCas9.TAD activators, guided by gRNAs complementary to promoter elements, induce strong transcriptional activation on episomal targets in plant cells. Moreover, our data suggest that the dCas9.SRDX repressor and the dCas9.EDLL and dCas9.TAD activators are capable of markedly repressing or activating, respectively, the transcription of an endogenous genomic target. Our data indicate that the CRISPR/dCas9: TFs DNA targeting system can be used in plants as a functional genomic tool and for biotechnological applications Full Product DetailsAuthor: Baazim HatoonPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.50cm , Length: 22.90cm Weight: 0.141kg ISBN: 9783659611230ISBN 10: 3659611239 Pages: 88 Publication Date: 23 September 2014 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |