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Author:   Tudor A. Fulga ,  David J. H. F. Knapp ,  Quentin R. V. Ferry
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2162
Weight:   0.758kg
ISBN:  

9781071606865

ISBN 10:   1071606867
Pages:   286
Publication Date:   15 September 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experiments.- Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer.- Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells.- Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNA.- ‘Split-and-Click’ sgRNA.- Chimeric DNA-RNA Guide RNA Designs.- Harnessing tRNA for Processing Ability and Promoter Activity.- Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA–ncRNA Expression Constructs.- Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs.- Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a.- Methods for Measuring CRISPR/Cas9 DNA Cleavage in Cells.- In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR/Cas9 Systems.- Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq.- Detection of CRISPR/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral Vector.- Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method.

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