Overview

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. 

Full Product Details

Author:   Sebastian Dworkin
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2403
Weight:   0.625kg
ISBN:  

9781071618493

ISBN 10:   1071618490
Pages:   313
Publication Date:   16 December 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development Michael Gruss and Ann K. Corsi  2. Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial DevelopmentNishanthi Mathiyalagan and Sebastian Dworkin  3. Elucidation of Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-Mount In Situ Hybridization Joshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam   4. Visualization of the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining Joshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam   5. Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue M. E. de Vries and S. Dworkin   6. Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion Jack D. Morgan and Jeremy B. A. Green   7. Single cell RNA-seq: Cell Isolation and Data Analysis Val Yianni and Paul T Sharpe   8. Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9 Priyanka Kumari, Morgan Sturgeon, Gregory Bonde, and Robert A. Cornell   9. Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures Mustafa Mian, Jenny Tan, Robin Yong, Ruth Williams, Agatha Labrinidis, Peter J. Anderson, and Sarbin Ranjitkar   10. Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull Jenny Tan, Agatha Labrinidis, Ruth Williams, Mustafa Mian, Peter J. Anderson, and Sarbin Ranjitkar   11. Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing Marco Osterwalder, Stella Tran, Riana D. Hunter, Eman M. Meky, Kianna von Maydell, Anne N. Harrington, Janeth Godoy, Catherine S. Novak, Ingrid Plajzer-Frick, Yiwen Zhu, Jennifer A. Akiyama, Veena Afzal, Evgeny Z. Kvon, Len A. Pennacchio, Diane E. Dickel, and Axel Visel   12. Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton Daniela M. Roth, Lakshmi Puttagunta, and Daniel Graf 13. Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development Jacob I. Reynolds, Ross A. Vitek, Peter G. Geiger, and Brian P. Johnson   14. Ex Vivo Culture of Human Cranial Suture Cells Peter J. Anderson   15. Scaffolds for Use in Craniofacial Bone Regeneration Katherine R. Hixon, Christopher T. Eberlin, Meghana Pendyala, Angela Alarcon de la Lastra, and Scott A. Sell   16. Mandible Explant Assay for The Analysis of Meckel’s Cartilage Development Sophie Wiszniak and Quenten Schwarz   17. High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models A. Huysseune, M. Soenens, J.-Y. Sire, and P.E. Witten   18. Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration Emma L. Moore and Paul A. Trainor   19. Salivary Gland Development In Culture Marcia Gaete, Tathyane H. N. Teshima, Lemonia Chatzeli, and Abigail S. Tucker   20. Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis Sarah Constantine and Piotr Niznik

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