Overview

Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.

Full Product Details

Author:   Thomas Muller-Reichert (Professor of Structural Cell Biology, Technische Universitat Dresden, TU Dresden, Germany) ,  Paul Verkade (The University of Bristol, Bristol, UK)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   1.060kg
ISBN:  

9780128220580

ISBN 10:   0128220589
Pages:   456
Publication Date:   08 March 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Workflow (dis)-advantages / spiderweb Ori Avinoam 2. Serial section LM + EM Erin M. Tranfield 3. Platinum clusters as CLEM probes Paul Verkade 4. Correlative Light Electron Microscopy with a transition metal complex as a single probe Paul Verkade 5. Detection EDX + EELS Ben N. G. Giepmans 6. SEM-TEM-SIMS Louise Jensen 7. HPF-CLEM Xavier Heiligenstein 8. A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes Thomas Müller-Reichert 9. Three-dimensional on-section correlative light and electron microscopy of large cellular volumes using STEM tomography Korbinian Buerger 10. Accelerated procedure for approaching and imaging of optically branded ROI in tissue Natalia V. Gounko 11. Volume-CLEM Kedar Narayan 12. Cryo SOFI CLEM Rainer Kaufman 13. Cryo fluo after Cryo lamella Petr Chlanda 14. Super resolution CLEM Sílvia Pujals 15. FIB-SEM + software Allon Weiner 16. Correlia, CLEM software Matthias Schmidt 17. Correlated Multimodality Imaging beyond CLEM: From in-vivo Preclinical Imaging to ex-vivo Microscopy Andreas Walter 18. Community efforts Paul Verkade

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Author Information

Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM. TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019. He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.

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