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Author:   Helena J. Maier ,  Erica Bickerton
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2020
Volume:   2203
Weight:   0.751kg
ISBN:  

9781071608999

ISBN 10:   1071608991
Pages:   278
Publication Date:   25 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Coronaviruses: An Updated Overview of Their Replication and Pathogenesis.- Discovery of Novel Coronaviruses in Rodents.- Detection and Discovery of Coronaviruses in Wild Bird Populations.- Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV).- Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing.- Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study.- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation.- The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation.- Isolation and Propagation of Coronaviruses in Embryonated Eggs.- Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures.- Quantification of Coronaviruses by Titration In Vitro and Ex Vivo.- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses.- In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline.- Proximity Labeling for Identification of Coronavirus-Host Protein Interactions.- Using Yeast to Identify Coronavirus-Host Protein Interactions.- Determining How Coronaviruses Overcome the Interferon and Innate Immune Response.- Ribopuromycylation in Coronavirus-Infected Cells.- Visualizing Coronavirus Entry into Cells.- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.

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