Overview

Leading investigators describe in detail their best techniques to study the cellular and molecular biology of connexins, as well as physiological properties. These forefront methods are specifically designed to investigate the life of connexins from biosynthesis to assembly into gap junction channels, and also include powerful assays to perform functional studies on these routes of cell cell communication. Also discussed are the many specific theoretical aspects of connexin biology and physiology. Cutting-edge and highly practical, Connexin Methods and Protocols offers laboratory researchers a comprehensive collection of proven methods for studying the role of connexins in intercellular communication and human genetic disorders, as well as finding promising new therapeutics for those disorders.

Full Product Details

Author:   Roberto Bruzzone ,  Christian Giaume
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   154
Dimensions:   Width: 15.20cm , Height: 3.00cm , Length: 22.90cm
Weight:   0.881kg
ISBN:  

9780896036581

ISBN 10:   0896036588
Pages:   491
Publication Date:   01 December 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Tools to Study Connexins.- Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques.- Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions.- Purification of Gap Junctions.- Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions.- Connexins/Connexons.- Biochemical Analysis of Connexon Assembly.- Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells.- Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction.- Use of Retroviruses to Express Connexins.- Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides.- Transfection and Expression of Exogenous Connexins in Mammalian Cells.- Assays For Function.- Assaying the Molecular Permeability of Connexin Channels.- Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins.- Mutagenesis to Study Channel Structure.- Dual Patch Clamp.- Determining Ionic Permeabilities of Gap Junction Channels.- Fluorescence Recovery After Photobleaching.- Capture of Transjunctional Metabolites.- Physiology and Biology of Connexins.- The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes.- Exploring Hemichannel Permeability In Vitro.- Inducing De Novo Formation of Gap Juntion Channels.- Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain.- Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds.- Biochemical Analysis of Connexin Phosphorylation.- How to Close a Gap Junction Channel.

Reviews

This book will be invaluable to those in the connexin field and a fascinating read and useful text to those new to this area of research. -Molecular Biotechnology

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