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Author:   Fabio Mammano ,  Mauricio Retamal
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2024
Volume:   2801
ISBN:  

9781071638415

ISBN 10:   1071638416
Pages:   200
Publication Date:   06 April 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Purification, Reconstitution, and Functional Analysis of Connexin Hemichannels.- Expression and Function of Connexins in Extracellular Vesicles.- Expression, Purification, and Nanodisc Reconstitution of Connexin-43 Hemichannels for Structural Characterization by Cryo-Electron Microscopy.- Molecular Dynamics Simulation of Permeation through Connexin Channels.- Spatial and Temporal Localization of Connexins in Cells Using Confocal Microscopy.- Assessment of Connexin43 Hemichannel Functionality Based on Cytosolic Uptake of Yo-Pro1.- A Dye Uptake Assay to Measure Large-Pore Channel Activity in Trypanosoma cruzi.- Measurement of Ca2+ Uptake through Connexin Hemichannels.- Evaluation of Connexin Hemichannel Activity In Vivo.- Protocol for the Study of Connexin and DNA Interactions.- Protocol for the Electrophysiological Characterization of Endogenous Pannexin 1 Intercellular Cell-Cell Channels.- Generation of Connexin-Expressing Stable Cell Pools.- A Protocol for the Automated Assessment of Cutaneous Pathology in a Mouse Model of Hemichannel Dysfunction.- Measuring Connexin Hemichannel Opening in Response to a InsP3-Mediated Cytosolic Ca2+ Increase.

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