Overview

B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement's mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of its function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies. Comprehensive and cutting-edge, Complement Methods and Protocols offers today's basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation.

Full Product Details

Author:   B. Paul Morgan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   150
Dimensions:   Width: 15.20cm , Height: 1.50cm , Length: 22.90cm
Weight:   1.220kg
ISBN:  

9780896036543

ISBN 10:   0896036545
Pages:   259
Publication Date:   30 March 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

The Complement System:An Overview.- Purification of Complement Components, Regulators, and Receptors by Classical Methods.- Immunoaffinity Methods for Purification of Complement Components and Regulators.- Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates.- Measurement of Complement Lysis of Nucleated Cells.- Functional Assays for Complement Regulators.- Immunochemical Measurement of Complement Components and Activation Products.- Complement Deposition in Tissues.- Complement Regulators and Receptors in Tissues.- Measurement of C3 Fragment Deposition on Cells.- Screening for Complement Deficiency.- C1-Inhibitor: Antigenic and Functional Analysis.- Autoantibodies to Complement Components.- Allotyping of Complement Components.- Complement and Immune Complexes.- Knocking Out Complement Genes.- Inherited Complement Deficiencies in Animals.

Reviews

recommended for inclusion in biomedical research libraries, both corporate and academic. - E-Streams

recommended for inclusion in biomedical research libraries, both corporate and academic. - E-Streams

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